Linear puromycin marker

Author: vudp

August undefined, 2024

Nettet美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品， Nettet23. des. 2008 · Construction of the insertion vectors pINS. We have used the backbone of the pUNI-10 plasmid [12, 13] (Fig 1, 2) for construction of the insertion vectors pINS-Puro, pINS-Neo and pINS-Blast. pUNI-10 contains the R6Kγ origin of replication [14, 15] and the LoxP site [] recognized by Cre recombinase [].R6Kγ origin is active only in E. coli …

Bidirectional promoter vectors - Takara Bio

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96871 NettetLinear Selection Markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer Linear Selection Markers for either hygromycin resistance (Linear Hygromycin Marker) or puromycin resistance (Linear Puromycin Marker). These markers are short, purified linear DNA fragments … tweety bird and sylvester cartoon videos

Puromycin - an overview ScienceDirect Topics

Nettet联系人：Dr.Xu, Biovector NTCC Inc. 电话： 400-800-2947 工作QQ:1843439339 (微信同号) 邮件： [email protected]. 手机： 18901268599. 地址：北京. 已注册. 详细信息. 咨询记录 (共 0 条) Linear Puromycin Marker. NettetPlasmid vectors using puromycin resistance as a selective marker have been developed for many methanogenic Archaea. The current vectors are constructed either with … NettetManufacturer: Clontech Labs 3P 631626. Linear Puromycin Marker, Size: 2microgram, it is a short ds DNA fragment consisting of the puromycin resistance gene regulated by … tweety bird apparel

Evaluating the influence of selection markers on obtaining …

Nettet28. aug. 2024 · 上海市闵行区动物疫病预防控制中心，上海201109) 摘要:应用Tet-on 系统和H1299细胞，建立了p53诱导表达的H1299细胞系p53-Tet H1299 , p53表达受强力霉素 ( doxycycline. Dox) 调控，存在一定的剂量依赖性诱导表达的p53 蛋白有很好的核定位能力和转录活性，能显著上调p21 ... Nettet25. jan. 2014 · We test a puromycin- N -acetyltransferase gene ( pac) as a selection marker in ciliates. The pac gene was altered to the most preferred codon-usage in T. … tweety bird and granny imagesNettet2 µg Linear Hygromycin Marker (also sold separately as Cat. No. 631625) 2 µg Linear Puromycin Marker (also sold separately as Cat. No. 631626) 100 rxns Xfect™ transfection reagent (also sold separately as Cat. No. 631320) 50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. No. 631105) tweety bird and sylvester coloring pages

"NettetLinear Puromycin Marker. 2 μg. 319,000원. pTRE-Tight는 response plasmid로 최적화된 TRE 기반의 promoter인 PTight를 가지고 있어 정밀하게 목적 유전자의 유도 발현을 조절할 수 있다 (Figure 1). PTight는 낮은 basal expression과 최고의 inducible expression을 제공한다. 또한, pTRE-Tight와 Linear ... " - Linear puromycin marker

Linear puromycin marker

Different properties of polysialic acids synthesized by the ...

Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a ... NettetA coleção “Ciências do esporte e educação física: Pesquisas científicas inovadoras, interdisciplinares e contextualizadas 2” é uma obra que tem como foco principal a discussão científica por intermédio de trabalhos diversos que compõem seus capítulos.

Did you know?

NettetLinear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly … NettetLinear Selection Markerは、トランスフェクションの安定性が要求されるあらゆる発現ベクターとのコトランスフェクションに理想的である。本マーカーは精製された短い …

http://www.biovector.net/product/45324.html Nettet联系人：Dr.Xu, Biovector NTCC Inc. 电话： 400-800-2947 工作QQ:1843439339 (微信同号) 邮件： [email protected]. 手机： 18901268599. 地址：北京. 已注册. 详细信息. 咨 …

NettetPuromycin (16) is characterized by 3′-amino-3′-deoxy adenosine and tyrosine moieties combined with a number of N- and O-methylations.Its biosynthetic pathway in the … Nettethuman lung carcinoma cells are best suited for titering vectors carrying puromycin selection marker, whereas Hepa mouse hepatoma cells for those with blasticidin selection marker. page 2 ... and the linear range and sensitivity of the assay. Example: Standard Curve y = 11286x + 1048.7 R2 = 0.9723 0 5,000 10,000 15,000 20,000 25,000 30,000

NettetI would personally clone a puromycin selection marker in for fast selection. You could clone a 2A FMDV sequence downstream your GFP followed by PURO.This way you …

NettetLinear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Line ... tweety bird baby blanketNettetDetails. Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 … tweety bird and friends vhsNettet我们提供具有潮霉素（Linear Hygromycin Marker）或嘌呤霉素（Linear Puromycin Marker）抗性的线性选择标记。这些标记是由标记基因、SV40启动子和SV40多聚腺 … tweety bird apronNettetLinear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly … tweety bird baby clothesNettetThermo Fisher ptetone construct plus linear puromycin marker Ptetone Construct Plus Linear Puromycin Marker, supplied by Thermo Fisher, used in various techniques. … tweety bird baby shower decorationsNettet9. jan. 2024 · Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb … tweety bird baby showerNettet27. mai 2024 · In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE-Tight-Gene X plasmids should be cotransfected with the Linear Hygromycin Marker (#631625 or #6202-1, not … tweety bird a tale of two kitties